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Image Search Results
Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology
Article Title: Inhaled house dust programs pulmonary dendritic cells to promote type 2 T-cell responses by an indirect mechanism
doi: 10.1152/ajplung.00256.2015
Figure Lengend Snippet: HDE-mediated conditioning of cDCs does not involve IL-25, IL-33, or thymic stromal lymphopoietin (TSLP). A: FL-cDCs prepared from C57BL/6 mice were treated with BALF-HDE from WT (open bars) or Il33−/− mice (solid bars) for 24 h and then cultured with naïve CD4+ OT-II cells in the presence of OVA peptide to induce Th cell differentiation. Bars represent the means ± SD of duplicate wells. Data are from 1 experiment, representative of 2 experiments. B and C: FL-cDCs were treated with BALF-HDE in the presence of neutralizing anti-IL-25 (B, solid bars), anti-TSLP (C, solid bars), or isotype control antibodies (B and C, open bars) for 24 h and then cultured with naïve CD4+ OT-II cells in the presence of OVA peptide to induce Th cell differentiation. Bars represent the means ± SD of duplicate wells. Data are from 1 experiment, representative of 2 experiments. **P < 0.01.
Article Snippet: In some experiments, 10 μg/ml of neutralizing polyclonal anti-mouse TSLP (R&D Systems Minneapolis, MN) ( 36 ), neutralizing monoclonal
Techniques: Cell Culture, Cell Differentiation